Threonine as a carbon source for Escherichia coli
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چکیده
منابع مشابه
Properties of threonine deaminase from a bacterium able to use threonine as sole source of carbon.
A threonine deaminase susceptible to inhibition by isoleucine was purified over 3,000-fold from extracts of Pseudomonas multivorans, a bacterium able to use threonine or alpha-ketobutyrate as sole source of carbon and energy. The enzyme was characterized with respect to molecular weight, dissociation to subunits, and apparent affinities for threonine, isoleucine, and several other ligands. Cert...
متن کاملThreonine as a perferred source of 2-carbon units for lipid synthesis in Trypanosoma brucei.
Culture forms of Trypanosoma brucei S42 were maintained in defined media by serial subculturing (Cross & Manning, 1973; Klein et al., 1975). Under these conditions considerable quantities of L-threonine were metabolized and equimolar concentrations of glycine and acetate were excreted. Glycine was identified by using an amino acid analyser and acetate was characterized as the p-bromophenacyl de...
متن کاملDisruption of the fucose pathway as a consequence of genetic adaptation to propanediol as a carbon source in Escherichia coli.
In Escherichia coli, L-fucose is dissimilated via an inducible pathway mediated by L-fucose permease, L-fucose isomerase, L-fucose kinase, and L-fuculose 1-phosphate aldolase. The last enzyme cleaves the six-carbon substrate into dihydroxyacetone phosphate and L-lactaldehyde. Aerobically, lactaldehyde is oxidized to L-lactate by a nicotinamide adenine dinucleotide (NAD)-linked dehydrogenase. An...
متن کاملSystems metabolic engineering of Escherichia coli for L-threonine production
Amino-acid producers have traditionally been developed by repeated random mutagenesis owing to the difficulty in rationally engineering the complex and highly regulated metabolic network. Here, we report the development of the genetically defined L-threonine overproducing Escherichia coli strain by systems metabolic engineering. Feedback inhibitions of aspartokinase I and III (encoded by thrA a...
متن کاملThe fate of ribosomes in Escherichia coli cells starved for a carbon source.
The disappearance of ribosomes in Escherichia coli cells starved for a carbon source was studied. We used a series of mutants, some of them lacking in ribonuclease I(RNase I, EC 2.7.7.17), and other containing various combinations of modified polynucleotide phosphorylase (PNPase, EC 2.7.7.8) and modified ribonuclease II (RNase II, EC 3.1.4.1). RNA was prepared from the starved mutant cells and ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1981
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.145.3.1150-1153.1981